The central area remains just pristine beach. Instead, each hilly end of this beach has been built up with condos, hotels, vacation rental homes, shops, and restaurants. But Flamingo has no real town center and the land behind its beach is sheltered from development because of a large mangrove forest. Most destinations are either set back from a main beach or centered around a downtown square or soccer field. Playa Flamingo has a different setup than other towns in Costa Rica. Juan Santamaria International Airport (SJO) in San Jose is about five hours away.Īll roads getting to Playa Flamingo are relatively flat and paved. The closest major airport is Daniel Oduber Quiros International Airport (LIR) in Liberia, about one hour away. Playa Flamingo is located in Costa Rica’s Guanacaste Province along the northern Pacific coast. In this post, we’ll share everything you need to plan a visit to Playa Flamingo. Famous for its small sandy cove, this highly developed beach town provides travelers with high-end amenities and stunning sea views. This, essentially, is what to expect in Playa Flamingo, Costa Rica. A beach umbrella flutters with the sea breeze. Your condo or vacation rental sits perched on a rocky point nearby. Your search is over! Use our advanced search filters to quickly identify the right properties and contact us when you’re ready to learn more.Imagine laying on a fluffy white-sand beach, backed by quiet mangroves. Did we mention that our technology provides the unique advantage for customers to browse properties on our MLS style website featuring all the inventory of the Coldwell Banker® Costa Rica network? If you’re searching for Flamingo Costa Rica properties, and want to make sure you’re seeing the best available selection, you found the right place. We offer our customers unparalleled service, technology, and local expertise. Real Estate in Flamingo Costa Rica Coldwell Banker® Costa Rica features the most trusted network of franchise partners in Costa Rica. The area has everything you’re looking for including quick transportation from North America with the international airport just a short 1 hour drive from the beaches of Flamingo. Did we mention the beautiful beaches? It’s no wonder why the area is so popular and buyers can choose from an outstanding variety of real estate in Flamingo Costa Rica including beachfront, luxury, golf course, mountain retreat, and ocean view. The region features exceptional sport-fishing, surfing, dining, golf, and other activities. Playa Flamingo is a premier destination for buyers and travelers seeking a world-class location for fun and relaxation. Your search is over! Use our advanced search filters to quickly identify the right properties and contact us when you’re ready to learn more. Flamingo Costa Rica Real Estate Playa Flamingo is a premier destination for buyers and travelers seeking a world-class location for fun and relaxation.
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Yes, God, help us to live with that shield of faith. In all circumstances, take up the shield of faith with which you can extinguish all the flaming darts of the evil one. Help us to faithfully share the gospel of peace, to go with our feet into the world today and share the gospel of peace. How do we know that’s the battle that’s happening today? There are people around us who don’t know the gospel of peace, that the spiritual forces of evil and heavenly places are trying to keep in the dark. Having put on the readiness given by the gospel of peace, Scott Mayer, our feet, be ready to share the gospel of peace today. Help us to live clothed in his righteousness and his shoes for your feet. Yes, God help us to live with the righteousness of Jesus today. Ephesians 6:11–18 Asks God to Help Us Live Righteously We pray for truth to guard our minds and our hearts in Christ and having put on the breast plate of righteousness. We want take up your whole armor ultimately to stand firm in the final day, stand therefore having fastened on the belt of truth. Therefore, take up the whole armor of God, that you may be able to withstand in the evil day, and having done all to stand firm. It’s spiritual forces of evil and the heavenly place is not flesh and blood. God help us to see that’s our battle today. Help us to see beyond the natural, to the spiritual, to spiritual forces of evil and the heavenly places who are working to harm us, to harm others around us today, who want us to experience hurt and pain, and who are working to destroy us. Oh God, open our eyes to the spiritual battle around us. Ephesians 6:11–18 Asks God to Open Our Eyes For we do not wrestle against flesh and blood, but against the rulers, against the authorities, against the cosmic powers over this present darkness against the spiritual forces of evil and the heavenly places. Give us wisdom and discernment, power, strength, everything we need to stand against the schemes of the Devil. We want to live with this armor on today. We want to stand against the schemes of the Devil today and every day in our lives. And I think it’ll be pretty obvious when I’m reading from this passage and praying according to this passage. To that end, keep alert with all perseverance, making supplication for all the saints.Įphesians 6:11–18 is the expectation from Paul to put on the whole armor of God. In all circumstances take up the shield of faith, with which you can extinguish all the flaming darts of the evil one and take the helmet of salvation, and the sword of the Spirit, which is the word of God, praying at all times in the Spirit, with all prayer and supplication. Stand therefore, having fastened on the belt of truth, and having put on the breastplate of righteousness, and, as shoes for your feet, having put on the readiness given by the gospel of peace. Therefore take up the whole armor of God, that you may be able to withstand in the evil day, and having done all, to stand firm. For we do not wrestle against flesh and blood, but against the rulers, against the authorities, against the cosmic powers over this present darkness, against the spiritual forces of evil in the heavenly places. Put on the whole armor of God, that you may be able to stand against the schemes of the devil. This gives you the opportunity to enjoy a match in HD, if your internet connection is strong enough, or watch it in a lower quality stream if your connection speeds are slow. Each match is available in multiple viewing streams. Sportsurge is an aggregator that allows NHL fans to watch all the NHL games in one place. Willy Daunic and Chris Mason and have the call on the television side, while Pete Weber and Hal Gill will broadcast on 102.5 The Game and the Predators Radio Network.Reddit NHL Streams Live Watch NHL on Sportsurge Streams puck drop begins with Predators Live on Fox Sports Tennessee at 1:30 p.m. All donations will benefit Second Harvest Food Bank.Ĭoverage for this afternoon's 2 p.m. The most needed items include: canned chicken and tuna, soups, stews, canned veggies and fruit, cereal, peanut butter, pasta and rice. The Preds and Second Harvest Food Bank will be collecting non-perishable food items and monetary donations on the plaza at Bridgestone Arena before today's game. and the first 5,000 children in attendance will receive Catfish magnets presented by Captain D's. A Plaza Party, presented by Lee Company, begins at 11 a.m. 20 brought his career total to nine points (7g-2a) in nine contests.īridgestone Arena doors open at noon CT ahead of today's 2 p.m. Viktor Arvidsson is on a three-game point streak (4g-1a) against Edmonton, and his goal against the Oilers on Oct. Subban has tallied 18 points (3g-15a) in 17 career games against Edmonton, including five points (1g-4a) in his last five games. Ryan Johansen has six points (1g-5a) in his last eight games against the Oilers dating back to March 14, 2016. He set a Preds franchise record for most saves in a shutout when he posted 46 vs. Juuse Saros has never allowed a goal against the Oilers, stopping all 77 shots he's faced in two starts. The Preds have shut out the Oilers twice in their last four meetings, including a 3-0 blanking on Oct. During the streak, the Preds have outscored the Oilers 40-15 and limited them to two-or-fewer goals 12 times, including five shutouts. Nashville's streak began with a 4-1 win over Edmonton on Oct. Nashville has won 13 consecutive games against Edmonton, its longest active winning streak against an opponent. The Predators are 43-23-(3)-3 all-time against the Oilers, including a 21-12-(3)-0 record at Bridgestone Arena. Cam Talbot has started all eight games for Edmonton to this point, but goaltender Mikko Koskinen is expected to get the start this afternoon for the Oilers. Connor McDavid paces Edmonton with six goals and 14 points, followed by Ryan Nugent-Hopkins with 11 points and Leon Draisaitl with eight. Forsberg and Arvidsson have each tallied on seven occasions, while Craig Smith has four goals for Nashville.Įdmonton has earned three out of a possible four points in their last two games after meeting the Preds last week, including a 4-1 win over Washington on Thursday. Yannick Weber, Viktor Arvidsson and Filip Forsberg also found the back of the net, while Saros made 33 saves in the victory.įorsberg continues to lead the club with 11 points, followed by his linemates, Arvidsson and Ryan Johansen, who have 10 points each. Kyle Turris scored the OT winner as the Preds topped the Devils, 4-3, on Thursday night. Video: Turris powers Predators to 4-3 overtime victory Rinne did skate during Friday's practice, and although Laviolette said there is no timetable for the goaltender's return, he did add that his presence on the ice is a positive sign. Saros, who blanked the Oilers last week, could face them again as goaltender Pekka Rinne remains on Injured Reserve. "Specialty teams have been working to get on track, and they really came through in the third period and won us a hockey game. "Our guys showed up and played hard, and I liked the fact we kept fighting back in that game," Predators Head Coach Peter Laviolette said following the win. Nero: (L) water blunt that reduce enemy's water resist. Nikeh: X2 (L) water slash, reduce enemy's water resist which pairs well with other water attackers. If you have them, use them.Ĭyrus: X2 (L) water slash, VC that reduce power and physical resist on enemy.Īkane: X2 (L) fire slash, VC that buff physical resist for your team. I will not state the obvious like Mighty, Mariel, Annabelle and Shion. resist up on a character with taunt and let them take the hit. Her strongest attack can easily deals 5k+ damage unmitigated) Luckily, she only attacks a single target, so you can simply stack p. All her attack deals heavy physical damage, so power debuff or physical resistance buff is a must or she will one shot any characters with her stronger attack. Mage that have no access to at least L single target spells do pitiful damage and should not be considered as main dps (sorry Lele). She has high magical defense, so your main dps should consist of mostly water or fire physical attacker. A tl dr version: bring a tank with taunt, a healer and all of your strongest fire and water attacker, preferably with multi hit abilities and physical resist buff/ power debuff on enemy.Īzami is weak to fire and water, and start off resisting wind, but later earth and all non elemental physical attack (like slash, pierce and blunt) as well as you progress through higher difficulties. Do note that this guide contains heavy spoilers for the fight, so if you want to do it blind, stop reading now. Azami's chance encounter is just right around the corner, and this guide is to let you know what character you should be leveling up to beat her lvl80 battle. Writer(s): Grant Perez, Linden Berelowitz, Daniel HenigLyrics powered by www.musixmatch. Huh Dance Yeah, I said let's dance the night away Baby, we could try to Make the world spin slower We could take our time and Get to know each other over cherry wine Oh, I, dance the night away Baby we could try to Make the world spin slower We could take our time, uh Know it's too soon to say you're mine Let's have a little drink tonight And. Verse 1 Why you standin' all by yourself Those shoes were made for dancin' with someone else Why don't we move over to that empty space I'll bet you twenty bucks I'll put a smile on your face. Dynamite I can show while we dance the night away Baby, we could try to Make the world spin slower We could take our time and Get to know each other over cherry wine I′ve been waiting on this dance Floor made for two babe Won′t you show me just what you can do? I don't wanna waste your time So please just have me for tonight? Huh Dance Yeah, I said let′s dance the night away Baby, we could try to Make the world spin slower We could take our time and Get to know each other over cherry wine Oh, I, dance the night away Baby we could try to Make the world spin slower We could take our time, uh Know it's too soon to say you′re mine Let's have a little drink tonight And get to know each other over cherry wine Why you standing all by yourself? Those shoes were made for dancing with someone else Why don′t we move over to that empty space? I bet you 20 bucks I'll put a smile on your face I know a place where we can Dance the night away Baby, we could try to Make the world spin slower We could take our time and Get to know each other over cherry wine, huh Well, it′s so soon to give up on tonight, uh I haven't had the chance to show you my moves and I I think you'd like to see why they would call me Mr. 0:00 / 3:04 Cherry Wine - grentperez (Lirik Lagu Terjemahan) DeLirik 5.48M subscribers Subscribe 1. Phase 1A - Polymerase Chain Reaction (PCR) Fresh tubes of P424 and P414 plasmids made using the miniprep kit. From here, the first iteration of the build process commenced.įigure 6. The plasmids were made using New England BioLab’s Monarch® Plasmid Miniprep Kit. The first step to produce both linearized backbones was creating plasmid DNA from each of the bacterial strains. The two strains were named P424 and P414, with P424 being a high copy version and P414 a low copy version. Tomko’s lab, based out of the FSU College of Medicine. The backbones originated from bacterial strains supplied by Dr. There were two versions of the linearized backbone created. We ensured that we were revising our workflow to optimize the effectiveness of each build iteration. Failures within the build stage were taken as an opportunity to improve upon our own designs. Within the engineering cycle, our team constantly fluctuated between the build and design stages to ensure that we had the best chance of bringing our ideas to life. Photograph courtsey of Nicole.īridging the gap between imagination and creation is much simpler said than done. All vectors designed and their specific modifications.įigure 5. Given every possible variation in assembly, there were 24 vectors designed: 6 expressing CTB1 alone, and 18 co-expressing CTB1 and CTB3, with each vector utilizing different promoter and copy number variations.Ĭonfigurations For Enzyme Expression Tuningįigure 4. Given every possible variation in assembly, there were 24 vectors designed: 6 expressing CTB1 alone, and 18 co-expressing CTB1 and CTB3, with each vector utilizing different promoter and copy number variations. Overlap based assembly of enzyme sequences and their regulatory elements, with (a) modular promoters and (b) copy number variant backbones. coli for cloning, and transformation in yeast for expression.įigure 3. Workflow of our assembly: Plasmid backbone linearization and DNA fragment addition, followed by HiFi assembly, transformation in E. Assays were then implemented for enzyme and product detection, including HA epitope tagging on CTB1, 6xHis tagging on CTB3 and analytical chemistry assays for product identification and quantification inspired by existing literature (2,3,4).įigure 2. Molecular cloning was done using NEB HiFi DNA assembly, allowing DNA fragments for coding sequences and regulatory elements to be interchanged by annealing purposefully designed overlaps. Choices of plasmid copy number and promoter strength were modulated to encourage maximum production in our chassis, resulting in several combinatorial designs. beticola and sequentially inserted them into shuttle vectors for cloning in E.coli and episomal expression in S. To do this, we selected the first two enzymes in the pathway – CTB1 and CTB3 – from the genome of C. The boxed regions show the enzymes and compounds of interest in our project, as well as the sequence of metabolite synthesis and our assembly. Cercosporin biosythesis pathway following recently proposed gene cluster expansion using computational methods. With the competition timeframe and information gathered from literature in mind (enzymes required and speculated in biosynthesis, intermediate stability, and product detection protocols), we decided to transgenically express the first two enzymes (CTB1 and CTB3) in the biosynthesis pathway as a proof of concept for the yeast mediated production of cercosporin.įigure 1. beticola from their yield limiting regulatory networks and artificially control expression in a more tractable organism – Saccharomyces cerevisiae. To eliminate these restrictions, our idea was to isolate the necessary genes in C. While biosynthesis of cercosporin in organisms such as Cercospora beticola has been made extremely efficient by natural selection, its regulation has not been fine-tuned for maximum yield: Many of the genes required for biosynthesis are regulated by factors that have not been fully elucidated, and the ones we know of such as sunlight, temperature, pH, and choice of media (1) make mass production challenging. Given the complexity of the molecule and its efficient biological synthesis due to natural selection, our team sought to leverage this existing efficiency to further adapt production. This is evidenced by the increases in fungal metabolite yields in yeast attained by modifying gene regulation, swapping protein domains, fusing enzymes and co-culturing with bacteria (10,11,12,13). Our design was inspired by the potential of synthetic biology to maximimze cercosporin yield in ways that its native fungal host and chemical synthesis cannot. #invasivespecies #invasive #plant #plants #botany #planttok #nativeplanttok #ecosystem #garden #nursery #fyp #plantstore #homedepot #lowes #sacramento #cali #california #sf #sj #sanjose #sanfrancisco #la #planttiktok #gardening #gardening101". French broom, Genista monspessulana: (Class A Weed) flowers 0.35 - 0.5 inches and in clusters of 4-10 at end of stem. It forms a large, spreading shrub that has small, oval, evergreen leaves and a profusion of bright yellow flowers. The seeds are produced in small, flattened pods 0.5 to 1 inch long. Reproduction is by seed and plants begin flowering from 18 months to 3 years of age. TikTok video from Jessie Dickson □ Home Depot selling more invasive plants. Origin: Mediterranean Invasive Yes high Habitat: Chaparral, woodland, disturbed areas Notes: This is one of the most aggressively invasive plants found on the Monterey Peninsula. French broom produces yellow, pea-shaped flowers in dense clusters of 4 to 10 flowers on short axillary shoots. Yellow to golden-yellow pea-like flowers (9-13 mm long) appear from May to. #invasivespecies #invasive #plant #plants #botany #planttok #nativeplanttok #ecosystem #garden #nursery #fyp #plantstore #homedepot #lowes #sacramento #cali #california #sf #sj #sanjose #sanfrancisco #la #planttiktok #gardening #gardening101ģ56 Likes, 60 Comments. Broom, Canary broom, Cape broom, Common broom, French broom, Madeira broom. Christensen)Ĭredit on screen with : ESO/L. Bacon (STScI)Įvaporating extrasolar planet, from Video (artist's impression)Ĭredit: ESA, Alfred Vidal-Madjar (Institut d'Astrophysique de Paris, CNRS, France) and NASA.Ĭredit: ESA/Hubble (M. Ring of rocky debris around a white dwarf star (artist’s impression)Ĭredit: NASA, ESA, STScI, and G. “Through a Computer Screen” by Raphael Olivier via KTSA Publishing and Universal Production Music The findings help describe the violent nature of evolved planetary systems and can tell astronomers about the makeup of newly forming systems. This is the first time astronomers have observed a white dwarf star that is consuming both rocky-metallic and icy material, the ingredients of planets.Īrchival data from NASA’s Hubble Space Telescope and other NASA observatories were essential in diagnosing this case of cosmic cannibalism. and Universal Production Music.Ī star’s death throes have so violently disrupted its planetary system that the dead star left behind, called a white dwarf, is siphoning off debris from both the system’s inner and outer reaches. “Unclaimed Space” by Peter Nickalls via Atmosphere Music Ltd. The background of panel 6 is a frame from an Illustris TNG simulation (Pillepich et al. Panels 1–5 show a “classical” slingshot scenario (e.g., Saslaw et al. Schematic illustration of the runaway SMBH scenario as an explanation of the key observed features. Image from paper “A candidate runaway supermassive black hole identified by shocks and star formation in its wake” by PI Pieter Von Dokkum et al. NASA’s Goddard Space Flight Center/Jeremy Schnittman It’s likely the result of a rare, bizarre game of galactic billiards among three massive black holes. The streamer is twice the diameter of our Milky Way galaxy. This potential supermassive black hole, weighing as much as 20 million Suns, has left behind a never-before-seen 200,000 light-year-long trail of newborn stars. But don’t worry, luckily this beast is very, very far away! There’s an invisible monster on the loose! It’s barreling through intergalactic space fast enough to travel from Earth to the Moon in 14 minutes. Tesseract by Cody Johnson and Gina Kouyoumdjian via Emperia Alpha Publishing, Emperia Beta Publishing, and Universal Production MusicĬomputer Representation of the Stellar Motions in the Core of M4:īlack Hole accreting material animation by Aurore Simmonet. How would they form, where would they hang out, and why do they seem to be so rare? The universe at large is flooded with supermassive black holes, weighing millions or billions of times our Sun’s mass and found in the centers of galaxies.Ī long-sought missing link is an intermediate-mass black hole, weighing in somewhere between 199 and 10,000 solar masses. It’s estimated that our galaxy is littered with 100 million small black holes (several times the mass of our Sun) created from exploded stars. Like intense gravitational potholes in the fabric of space, virtually all black holes seem to come in two sizes: small and humongous. Koka Media, Universal Publishing Production Music France, and Universal Production MusicĪstronomers using NASA’s Hubble Space Telescope have come up with what they say is some of their best evidence yet for the presence of a rare class of “intermediate-sized” black hole that may be lurking in the heart of the closest globular star cluster to Earth, located 6,000 light-years away. Koka Media, Universal Publishing Production Music France, and Universal Production Music Koka Media, and Universal Production Music “Claraboo” by Denis Levaillant, Jean-Marc Foltz. Killer Tracks, Open Note, and Universal Production Music “Cascades” by Air Jared, Sebastian Barnaby Robertson. Over a decade ago, when she was new to the job, she had a special project related to the Hubble Space Telescope and its fifth and final servicing mission. Paula Cain is one of the talented thermal blanket technicians who uses her skillful hands to correctly cover all sorts of spacefaring instruments. NASA’s Thermal Blanket Lab is a vital part of ensuring that the important equipment that we send into space remains protected from getting either too hot or too cold. The planner helps manage time and prioritize tasks, allowing users to stay on top of tasks and prioritize effectively. It is easy to use, customizable, and offers users a comprehensive view of their tasks and goals. My digital planner offers a range of features and benefits that make it an attractive option for busy professionals. ❌ This item is NOT designed to be printed ❌ This is a digital product and as such no physical product will be shipped. ❌ Not designed for use with mobile phones, except if only using the mobile phone for reference (message me if you are unsure about this), also not for kindles and Chromebooks Examples include Goodnotes, Notability, Samsung Notes, Collanotes, Xodo etc. ✅ The All-in-one digital planner has been designed to be used in conjunction with any PDF annotation app. ✅ Compatible with iPads, tablets (not Kindle, Remarkable 2 and a few others, please reach out before purchase), desktop (both Windows and Apple) and Laptop computers ✅ Compatible across Apple, Android, and Windows devices ✏️Monthly Calendars (January through December) with Monday start You’ll find many useful pages to help you with your monthly goals, budget, mood tracking, notes, ideas, and so much more. ✏️Blank Lined Paper for simple note-taking ✏️Tabs and Index with hyperlinks for easy navigation. ✏️Landscape format design, to fit much more on your screen. The digital planners we’ll cover in this article are all PDF documents that can be used in notetaking apps such as GoodNotes, Notability, and Noteshelf. However, digital planners have a lot more to offer, including added flexibility and customization, faster navigation via hyperlinks, and increased portability. Just as with a paper planner, you’ll be able to manage your schedule, keep up with all your to-dos, and track your habits. Digital planners help you streamline your workflow to stay productive. Looking for a fun and paperless way to stay organized in 2023?Ī digital planner may be exactly what you need. The tip is more pointy compared to Apple Pencils and hence, is more accurate by hardware. Their S-Pen for their Note series (and more recently their foldable lineup) also has a spring-loaded cap bottom that locks itself securely inside its dedicated phone slot. It features a rather glossy body slightly smaller than the Apple Pencil, splashed with a rainbow of colours with respect to its parent phone/tablet body. Samsung S-PenĪlthough Samsung has multiple models in its S-Pen range, most of the aesthetics try to be consistent. The tip is also a blunt tip that prioritizes touch sensitivity and is perfect for sketching. The 2nd generation replaced that design with magnetic charging on its flat side, thus making it much sleeker and easier to charge. The first generation also had a cap on the other end that housed the poorly implemented charging port. Apple PencilĪpple Pencil has a cylindrical pump design with a matte white finish all around that gives it a rather majestic feel and look.Īlthough the 1st generation Apple Pencil had a fully rounded design, the 2nd Gen fixed that issue with a flat side that provides better grip and doesn't roll off the table. The in-hand feel, weight, and material of the product make a significant impact on its utility. Apple Pencil vs Samsung S-Pen: Design and AestheticsĪesthetics is a key area to consider when you are in the market for a stylus pen. When you buy through links on my site, I may earn an affiliate commission at no extra cost to you. Judging purely on capabilities, the Apple Pencil has great software support and reliable hardware.īlinqBlinq is reader-supported. S-pen has a slight edge over the Apple Pencil due to its extra features and compatibility. Well, I seek to put an end to that and compare both the Samsung S-Pen and Apple Pencil and see who comes out on top: Experience or Tech prowess. I own both Apple Pencil and S-Pen, and I am constantly flooded with questions regarding which is the best overall stylus pen. They are well deserving of such recommendations too, due to their sheer dominance in their respective markets. Ask any tech enthusiast for a recommendation for a good stylus pen, and the two main answers you receive will be the Apple Pencil and the Samsung S-Pen. |
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